Contributing 6% of the global greenhouse effect，nitrous oxide (N₂O) is the third most important greenhouse gases in the world. According to the sampling data of the 33rd Chinese Antarctic Scientific Expedition in 2017, the tip of the Antarctic Peninsula (TAP) is divided into two study areas, the southern and the northern, with a boundary of South Scotia Ridge (SSR). N₂O concentration in the north was 14.6-21.6 μmol L-1, which is the source of atmospheric N₂O (10.84 μmol m-2 d-1). In contrast, the N₂O concentration in the south was lower (corresponding to the N₂O concentration range of 14.5-19.5 μmol L-1), which is a sink for N₂O (-0.71 μmol m-2 d-1). Combined with the TAP in 2017, the average air-sea flux of N₂O was estimated as 2.47 μmol m-2 d-1, was equal to 29.6% of 2012. Stratification and upwelling were the main factors controlling the changes in N₂O fluxes. Long-term observations are needed to understand and predict future changes in N₂O fluxes at the Antarctic Peninsula, especially when future sea ice melts.