Marine dinitrogen (N₂)-fixing diazotrophs can convert N₂ in seawater into bioavailable nitrogen (N), providing one half of the input of bioavailable N for the oceanic microorganisms. In order to facilitate global marine N₂ fixation research, this study compiled data from scientific literature of N₂ fixation activities over the last ten years on the basis of the MAREDAT database of N₂ fixation, and updated the data including abundance of nitrogen-fixing cyanobacteria (based on microscopic cell counts or qPCR assays targeting the nifH gene copies) and N₂ fixation rates a total of 16042 data points. The updated database includes six sub-databases, and data points from the database in this study almost covers the global ocean, which solved the problem of regional limitations in the MAREDAT database that lacked in field measurements in India ocean. However, data distribution in the database was still not uniform, and most field observations related to N₂ fixation activities were focused on the surface water in the Pacific Ocean and the Atlantic Ocean. Each sub-database in the paper is approximately logarithmic normal distribution, but the variance is quite large, and the non-zero values differ by 3 to 10 orders of magnitude. The geometric mean of the global ocean N2 fixation rate is 54 (30-105) Tg N yr^-1, which is similar to the estimated value of previous studies, but much lower than that of model simulation. And this discrepancy was probably related to the measurement method of N₂ fixation rates. Therefore, the study compared the  N₂ fixation rates obtained by the ¹⁵N₂ tracer assays, and found that the N₂ fixation rates measured by the ¹⁵N₂ bubble method was generally lower than the ¹⁵N₂ dissolution method. However, there are a lot of potential factors that affect the measurement of the  N₂ fixation rates, which may contribute to the bias of the N₂ fixation rates obtained by both ¹⁵N₂ tracer methods in field experiments. Hence, the underestimation of ¹⁵N₂bubble method is not a persistent trend. Overall, this database provides implications for future studies on the biogeochemical model, field sampling and meta-analyses of nitrogen-fixing activities.